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对于任何来源的DNA模板，FailSafe^TMPCR System都可以确保您得到稳定可靠的高保真度的PCR产物。FailSafe^TM PCR System每一次都可以忠实的扩增您的模板。

FailSafe^TM PCR System包括FailSafe^TM PCR Enzyme Mix和12种经过精心调配的酶特异性的适合于各种不同DNA序列的缓冲液FailSafe^TM PCR PreMixes。每一种FailSafe^TM PCR PreMix都含有dNTP，酶的最适buffer，经过仔细调节浓度的MgCl₂和含有Betaine的FailSafe^TM Enhancer。FailSafe^TM PCR Enzyme Mix是一种独特的耐热DNA聚合酶的混合物，这种酶能后有效的扩增复杂模板DNA并且有非常高的灵敏度和保真度。经过试验我们发现此混合酶的保真度至少是Taq DNA聚合酶的三倍。

The FailSafe PCR System is ideal for any PCR application, such as:
Multiplex PCR The ability of the FailSafe PCR System to amplify any template enables more reliable and consistent multiplex PCR (Figure 1).		Figure 1. Multiplex PCR of 5 exons of human CFTR. FailSafe™ amplified all 5 exons of the CFTR gene from as little as 1 ng of human genomic DNA.
PCR of “Difficult” Templates The FailSafe PCR System easily amplifies sequences with 85% GC or other sequences considered difficult with other PCR systems (Figure 2).		Figure 2. FailSafe™ PCR of 85+%-GC human fragile X gene. Fragile X gene was amplified from as little as 1 ng of total human genomic DNA.
High-Sensitivity PCR With the FailSafe PCR System, you can amplify a sequence present in only 1 molecule of DNA in the sample (Figure 3).		Figure 3. Detection of the cII gene in one copy of the phage lambda DNA genome.
PCR Any Sequence up to 20 Kb from Any Source The combination of the FailSafe Enzyme Mix, and the FailSafe PCR PreMixes, with our patented FailSafe PCR Enhancer with Betaine,* enable robust PCR of sequences and lengths that are not possible with other PCR systems (Figure 4).		Figure 4. FailSafe™ PCR of a range of different sequences and sequence sizes in phage, human and bacterial DNA. PCR products shown are up to 20 kb for lambda DNA, up to 21.5 kb for human DNA, and up to 18 kb for E. coli DNA.

FailSafe^TMPCR System采用了EPICENTRE拥有专利的PCR增强技术（PCR Enhancement Technology），简化了过去那种对于每一种模板和每一对引物都要都要不断调节反应成分已确定最适反应条件的过程。12种FailSafe^TM PCR PreMixes包含了使用于各种不同DNA序列的各种不同的反应条件。每一种PreMix代表了一个独特的PCR反应条件，并且包含了PCR反应所需的各种成分。您所需要做的就是将您的模板，引物和FailSafe^TM PCR Enzyme Mix混合，然后分别加到这12种FailSafe^TM PCR PreMixes中并在PCR仪上进行扩增反应。您会发现至少有一种FailSafe^TM PCR PreMixes是适合于您的模板和引物的。因此，在您以后用同样的引物和模板进行扩增时，您只需要订购这种FailSafe^TM PCR PreMix和FailSafe^TM PCR Enzyme Mix就可以得到稳定可靠的结果。

2，第一次PCR和以后每次PCR都能获得成功（Successful PCR，the first time and every time）;

Catalog No.	Size
FailSafe™ PCR PreMix Selection Kit
FS99060	60 U
Contents: FailSafe™ PCR Enzyme Mix and all 12 FailSafe™ PCR 2X PreMixes. Note: Each PreMix volume has been modified to match the Enzyme Mix volume.
FailSafe™ PCR System with PreMix Choice
FS99100	100 Units
Contents: Includes FailSafe™ PCR Enzyme Mix and choice of one FailSafe™ PCR 2X PreMix (see below).
FailSafe™ PCR System with PreMix Choice
FS99250	250 Units
Contents: Includes FailSafe™ PCR Enzyme Mix and choice of two FailSafe™ PCR 2X PreMixes (see below).
FailSafe™ PCR System with PreMix Choice
FS9901K	1000 Units
Contents: Includes FailSafe™ PCR Enzyme Mix and choice of eight FailSafe™ PCR 2X PreMixes (see below).
FailSafe™ PCR 2X PreMixes (PCR System inclusion only)
FSP995A	2.5 ml
FSP995B	2.5 ml
FSP995C	2.5 ml
FSP995D	2.5 ml
FSP995E	2.5 ml
FSP995F	2.5 ml
FSP995G	2.5 ml
FSP995H	2.5 ml
FSP995I	2.5 ml
FSP995J	2.5 ml
FSP995K	2.5 ml
FSP995L	2.5 ml
Contents: dNTPs, buffer, and various amounts of MgCl2 and FailSafe™ PCR Enhancer with Betaine. Note: This listing is only for indicating a choice of PreMixes included with the FailSafe™ PCR System (see above). The PreMixes are also available for purchase separately.
FailSafe™ Enzyme Mix Only
FSE51100	100 Units
FSE5101K	1000 Units
Note: We can only guarantee the failsafe nature of this system if the FailSafe Enzyme Mix is used with a FailSafe PCR 2X PreMix that was first determined using the FailSafe PCR PreMix Selection Kit (above).