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最新产品
>Epicentre 公司热点产品
Epicentre 公司热点产品
克隆相关产品
T4
DNA Ligase, Cloned
T4
DNA Ligase是一种广泛应用于DNA克隆的常用ATP依赖的连接酶。它共价连接限制性内切酶或者其它酶消化产生的双链DNA分子的平末端或者互补的粘末端的5´磷酸基和3´羟基。此酶对单链DNA分子没有连接酶活性。在连接反应结束后可以通过65°C 10分钟来灭活此酶。EPICENTRE拥有最好的T4
DNA Ligase。尽管如此，对于大多数的连接反应我们还是推荐使用Fast-Link™
DNA Ligation Kits，因为此试剂盒含有优化好的连接酶和其它组分，连接效率极高，并且连接反应仅仅需要5分钟。
应用：
1，
平末端或者互补的粘末端的双链DNA分子的连接（见图1）；
2，
修复双链DNA分子的切口。
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Figure 1. Ligation activity of EPICENTRE's T4 DNA Ligase.
T4
DNA Ligase at 10 U/µl was diluted sequentially in 10-fold
increments. One microliter of each dilution of T4 DNA Ligase was
incubated with Hind
III-cut lambda DNA at 16°C in 1X Reaction Buffer containing ATP.
Lane 1, kb ladder; Lane 2, no enzyme; Lane 3, 1 U/µl; Lane 4,
0.1 U/µl; Lane 5, 0.01 U/µl; Lane 6, 0.001 U/µl;
Lane 7, 0.0001 U/µl; Lane 8, 0.00001 U/µl. Note that
full enzyme activity is seen down to one one-thousandth unit; enzyme
activity is still apparent even at one ten-thousandth unit.
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Catalog No.
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Concentration
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Size
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T4 DNA Ligase, Cloned
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L0805H
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2 U/µl
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500 U
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L0810H
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2 U/µl
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1,000 U
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L0820H
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2 U/µl
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2,000 U
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LH805H
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10 U/µl
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500 U
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LH810H
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10 U/µl
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1,000 U
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LH820H
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10 U/µl
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2,000 U
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Includes 10X Reaction Buffer and a
separate 25 mM ATP Solution.
T4 DNA Ligase is also available in bulk. Please inquire.
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2006北京吉比埃生物技术有限公司